HGMS profiling, both during test development and following commercial deployment, requires proprietary workflows and operating procedures be established for each test. In addition, HGMS profiling requires significant technological capabilities in order to attain efficient, high throughput operations. These workflows, procedures, and capabilities are specific to each step of the testing process:
Specimen annotation is performed by experienced veterinary pathologists using a portal that enables them to review and annotate high resolution digital images, targeting cells of interest down to a 50 µm diameter. The portal provides our veterinary pathologists the ability to target specific groups of cells, distinguish between various cell types, and reject specimens based on a test’s stated specimen requirements.
The Bruker rapifleX™ is a matrix assisted laser desorption/ionization (MALDI) time of flight (TOF) mass spectrometer. Slides containing adhered tissue are directly inserted into the instrument, and a laser is rastered over designated tissue regions causing bombarded molecules to be ablated from the surface and ionized. Charged ions are then accelerated through the flight tube within the mass spectrometer toward the ion detector. Ions with a smaller mass/charge (m/z) ratio move faster through the flight tube than those with a larger m/z value. Because the flight tube is a known length, the “time of flight” correlates directly to the m/z ratio of the ion, which can be calculated using calibration standards of known molecular weight. The resulting mass spectrum displays all observed m/z ions that were detected within a specified mass range.